ABSTRACT
Aim To investigate the effect of Trillium tschonoskii Maxim ( TTM ) ethanol extract on hypoxia ischemia brain damage ( HIBD ) in neonatal rats and potential mechanisms. Methods Fifty healthy SD rats of 7 day-old were randomly divided into three groups:the sham operation group ( n=10 ) , the model group ( n=20 ) and TTM treatment group ( n=20 ) , which received 3-day intraperitoneal injection of normal saline or ethanol extract of TTM respectively. TTC staining and Nissl staining were performed to detect the cerebral ischemia area and neuronal death. Western blot was used to detect the expression of Bcl-2 and Bax. Re-sults The brain tissue of model group was slightly swollen, and white necrotic zone induced by ischemia occured on the right side of the brain, while the brain morphology of TTM treatment group was good. After TTC staining, ischemia zone was clearly seen on the right side of the brain in model group, while after TTM treatment, the size of ischemic zone was decreased. Compared with the model group , Nissl staining showed the neuronal cells increased in TTM treatment group. Western blot showed the expression of Bcl-2 protein in TTM group increased than that in HIBD model group ( P <0. 01 ) , while the expression of Bax protein de-creased ( P <0. 01 ) . Conclusion TTM therapy is beneficial for HIBD,which may be related to reducing neuronal apoptosis.
ABSTRACT
Objective To explore the immunization strategy at the endgame of poliomyelitis eradication in China.Methods To search the related study databases by the mean of evidence based medicine,and to investigate the update status of production and usage of polio vaccine in China.The general prospective analysis was conducted on aspects of the comparison of different immunization strategies,the feasibility of oral poliovirus vaccine(OPV)switching to Sabin strain inactivated poliovirus vaccine(Sabin-IPV)and the Sabin-IPV immunization schedule.Results The immunization strategy of OPV switching to IPV should be considered to choose in China because the domestic Sabin-IPV products will be registered soon and the switching feasibility is already provided with the conditions of police,technique,vaccine supply,and other guarantee.The routine immunization of Sabin-IPV can be carried out referring to the update OPV immunization schedule.Conclusion The study on immunization strategy at the endgame of poliomyelitis eradication should be further enhanced and the preparation of OPV switching Sabin-IPV should be considered in China.